FastQCFastQC Report
Wed 19 Jul 2017
G110_M4.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG110_M4.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10325607
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT13650.028.5243742
GATCGGA14550.027.3616731
CGTATGC15050.026.16225444
ATCGGAA17150.023.4706212
TCGTATG17000.023.16173743
AATCTCG17650.022.93890639
CGTCTGA18600.022.23112716
ATCTCGT19550.020.81917640
ACGTCTG19600.020.64821415
ACACGTC23950.017.17383813
TCTCGTA23300.016.90067741
CGGAAGA26400.016.74684
GCACACG25800.016.02740311
GACCAAT25900.015.97820935
ACGTTCG1552.6810812E-715.6087116
ACTGACC26350.015.53886132
CACACGT26700.015.48730212
TCGGAAG29000.015.01843
CACGTCT29900.013.90342814
ACCAATC29400.013.77655136
Produced by FastQC (version 0.11.2)