FastQCFastQC Report
Tue 8 Mar 2016
G110_M10.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG110_M10.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19231840
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA12150.018.6465911
TATCTCG12600.017.46333539
CTCGTAT15000.014.37088442
CGTCTGA19850.012.29879516
ACGTCTG19700.012.16943715
ATCGGAA18650.012.1480322
CGTATGC19900.011.38452144
TAGCTTA21650.010.37186834
CGGGCTA10300.010.0368891
CTCGAAT28750.09.9458981
TTATTCG11350.09.49788325
CGTACTA4401.0622898E-99.49662822
CCGCTAA9300.09.45984311
CGCGTAT4204.793037E-99.42674351
CGCCCAT29800.09.1525881
GTCGCAA5303.8198777E-119.1303051
GCTTATC26550.09.12067736
TCGGAAT22750.08.991852
GCCGGAT16400.08.9860631
TCGATAC11750.08.9856792
Produced by FastQC (version 0.11.2)