FastQCFastQC Report
Tue 8 Mar 2016
G110_M12.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG110_M12.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15762631
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC232070.14722795959633897TruSeq Adapter, Index 12 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA30450.036.1884041
CTCGTAT35200.031.99510242
CGTCTGA37850.029.9257616
ACGTCTG38400.029.78408215
CGTATGC38450.029.3460544
ATCGGAA37750.029.1913072
AATCTCG39950.028.86154439
ATCTCGT40600.027.85237140
GTAATCT49900.022.89613237
GCACACG51150.022.61818311
TCGTATG50700.022.42956543
CGGAAGA53750.021.9754054
TAATCTC52650.021.78274338
TGTAATC57400.019.98162336
TCTCGTA59750.019.14454741
ACACGTC61600.018.81717513
TCGGAAG61000.018.6066933
CTTGTAA67050.017.30309134
CACACGT68250.017.08038712
TCTGAAC70350.016.75620318
Produced by FastQC (version 0.11.2)