FastQCFastQC Report
Sat 19 Mar 2016
G110_M1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG110_M1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences250000
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGGCA550.004462899620.0084612
CCCAGAT1057.107958E-414.6728711
GGGAAGT1150.001413840313.3969691
AACTAAG1000.00874530413.19765956
GCCCAGT1250.002646637812.3252111
ACAATGT1250.002646637812.3252113
TTCACCA1950.00685903159.0240417
CTCAGCT2000.0084002358.8037221