FastQCFastQC Report
Sat 19 Mar 2016
G110_M2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG110_M2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences250000
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGC2660.10640000000000001TruSeq Adapter, Index 2 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGGGT207.843417E-444.0013961
TCGTATG408.28004E-943.99259643
CTCGTAT452.341767E-839.1045342
ATCTCGT553.4015102E-936.0011540
ACCGATG505.901711E-835.20816832
TATCTCG505.901711E-835.20816839
CACCGAT505.901711E-835.20816831
CCGATGT551.3635508E-732.00742333
ATCGGAA551.3635508E-732.0074232
GATCGGA551.3659519E-732.0010221
CGTATGC551.368353E-731.99461744
ATCCTAG300.00572990929.34013637
TCGGGGT300.00572990929.3401362
CGATGTA602.9236435E-729.34013634
GTCACCG703.558671E-828.29227329
CGCCCAT407.0151227E-427.5008741
TCACCGA655.887632E-727.08320430
TCTCGTA655.897946E-727.07778541
GCACACG756.963637E-826.4008411
ACACGTC701.1259544E-625.14365613
Produced by FastQC (version 0.11.2)