G110_M4.fastq FastQC Report

Summary

Basic Statistics
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Adapter Content
Kmer Content

Basic Statistics

Measure	Value
Filename	G110_M4.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	250000
Sequences flagged as poor quality	0
Sequence length	50
%GC	48

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

No overrepresented sequences

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTCTGA	45	4.0415827E-5	29.34948	16
CGGAAGA	45	4.0526393E-5	29.337736	4
CCGGGTT	30	0.0057322043	29.337734	1
CTCGTAT	30	0.005743427	29.326	42
GATCGGA	40	7.0102775E-4	27.504126	1
ATCGGAA	45	0.0013938969	24.448114	2
AATCTCG	45	0.0013955231	24.443224	39
TCGGAAG	50	0.002572525	22.0033	3
ATCTCGT	50	0.0025755165	21.9989	40
ACTGACC	50	0.0025755165	21.9989	32
ACGTCTG	60	0.0073726303	18.343424	15
GAGTAGT	60	0.0073896777	18.336084	4
CTTGGGA	85	0.0029681337	15.531741	1
AACTCCA	90	0.0043534017	14.663001	22
TCCAGTC	115	0.0014188931	13.390635	25
CAATCTC	100	0.008737949	13.199339	38
ACTCCAG	130	0.0035682074	11.843193	23
GGGTCTT	180	0.0035368742	9.779245	2