FastQCFastQC Report
Tue 8 Mar 2016
G110_M5.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG110_M5.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8573865
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC2855113.3300151098716855TruSeq Adapter, Index 5 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA306500.043.4154661
ATCGGAA307500.043.1248252
ACGTCTG313750.042.6430815
CGTCTGA314150.042.59504316
CTCGTAT312550.042.5491642
CGTATGC313700.042.4538144
ATCTCGT314950.042.16895740
CGGAAGA321950.042.0168954
GATCTCG317700.041.94855539
GCACACG321000.041.83657511
AGTGATC321150.041.2587536
TCGTATG323500.041.21020543
TCGGAAG321650.041.1746563
TCTCGTA327900.040.5948741
GTCTGAA332700.040.5617517
TCTGAAC333000.040.48557718
AGAGCAC334750.040.3680278
GTCACAC331800.040.27832429
ACACGTC334200.040.14606513
CACACGT336200.039.88060812
Produced by FastQC (version 0.11.2)