FastQCFastQC Report
Tue 8 Mar 2016
G110_M6.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG110_M6.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11824242
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC1850481.564988267324028TruSeq Adapter, Index 6 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA205950.042.8276021
TATCTCG207950.042.25017539
ATCGGAA209750.042.0319752
CGTCTGA212400.041.94986316
CTCGTAT208450.041.75628742
CGTATGC208750.041.65271844
ACGTCTG214100.041.63784815
GTCACGC214650.041.25929329
ACGCCAA215200.041.23468432
CGCCAAT215900.041.0194233
ATCTCGT214750.040.5893940
TCACGCC218350.040.5480530
AGTCACG219950.040.33275228
CGGAAGA224550.040.153254
GCACACG223650.040.01605211
CCAATAT222000.039.88121435
CAATATC222050.039.78301236
CACGCCA223350.039.74924531
ATATCTC222550.039.6914538
TCGTATG223200.039.0554943
Produced by FastQC (version 0.11.2)