FastQCFastQC Report
Tue 8 Mar 2016
G110_M8.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG110_M8.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11134284
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGC177630.15953428168349218TruSeq Adapter, Index 8 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA25700.036.2015271
CTCGTAT26600.033.2430742
ATCGGAA29050.032.0275422
CGTATGC27200.031.94266944
CGTCTGA30200.031.68136416
ACGTCTG30350.031.59768115
AATCTCG29200.030.59469839
ATCTCGT31250.028.23130640
GCACACG36200.026.3688411
CGGAAGA38350.025.4082954
ACACGTC46850.020.75123613
TCTCGTA43450.020.6568541
TCGGAAG47650.020.542063
TCGTATG43850.020.11534743
CACACGT50100.019.36110912
TTGAATC47550.018.88818636
AGAGCAC52650.018.6728768
TCGACCG4150.018.55873127
GTCTGAA55550.018.09341417
CACTTGA50000.017.69946133
Produced by FastQC (version 0.11.2)