FastQCFastQC Report
Tue 8 Mar 2016
G110_M9.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG110_M9.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17137277
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA11900.020.1467441
CTCGTAT15950.014.34290942
ACGTCTG18200.014.14014115
ACGATCA16500.013.60858532
ATCGGAA18150.013.4515682
CGTATGC17800.013.34567244
CGATCAG18650.012.86602633
CGTCTGA19450.012.55252816
CGCCCTA4000.012.0972861
CGCCTAT4600.011.9538411
ATCTCGT20150.011.68266140
TTATTCG11000.011.40033825
ACCGGTA20050.011.1947727
TCACGAT20650.010.97997330
CGCTAGT3502.4319888E-910.6833181
AGTCACG24850.010.45028428
GTCACGA22850.010.40235329
CGCCTGA12750.010.350621
TAAGGCG43050.010.26827619
AATTGCG21750.010.12082636
Produced by FastQC (version 0.11.2)