FastQCFastQC Report
Fri 6 Feb 2015
150126Wax_D15-380_NA_sequence.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filename150126Wax_D15-380_NA_sequence.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences191448252
Sequences flagged as poor quality0
Sequence length50
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCCGAG299050.016.22804644
TCTCCGA329750.014.6106143
ATCTCCG367650.013.202003542
GTCTCTT5656100.09.35177744
TCTCTTA4400250.08.22968944
TTATACA2201000.07.952052644
GATCGGA65650.07.8391021
CTTATAC2283750.07.577220444
TGTCTCT7711800.07.52615144
CACGACG27700.07.462849615
CGACGCT30150.07.220999717
CTCTTAT3427900.06.978558544
ACGACGC31800.06.84641616
CTGTCTC8856100.06.559973243
TCCGAGC116200.06.455840644
TATACAC2149650.06.30401944
TCTTATA2936550.06.151996644
GGCGGTC73750.05.9996138
ATCGGAA90300.05.94262272
AATTCGG72200.05.75743823
Produced by FastQC (version 0.11.2)