FastQCFastQC Report
Thu 12 Feb 2015
G111_M10.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG111_M10.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18889420
Sequences flagged as poor quality0
Sequence length50
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCGTA7950.015.49609943
CTCGTAT8100.014.39409344
GATCGGA9250.014.0279951
ACGACGC3102.3646862E-1112.7694816
ATCGGAA10050.012.6922342
CACGACG3409.094947E-1212.28961315
CGACGCT3653.45608E-1111.44755517
TATCTCG11100.011.10114441
CGTCTGA12150.010.86018816
ATCTCGT12200.010.2798242
ACACGTC13650.09.1833513
ACGTCTG16650.08.05710615
TCGGAAG17800.07.78389023
CACGTCT21450.06.56169714
CGGAAGA21150.06.34301334
GCACACG26850.05.73325411
TACACGA7854.332229E-65.60298213
Produced by FastQC (version 0.11.2)