FastQCFastQC Report
Thu 12 Feb 2015
G111_M11.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG111_M11.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17383543
Sequences flagged as poor quality0
Sequence length50
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCCGAG69400.026.27832244
TCTCCGA94200.019.33679443
ATCTCCG111600.016.42251442
TAGGACG31500.015.5700294
TCCGAGC21150.014.45795944
AGGACGT35950.013.3367585
GGACGTG34000.013.260626
GACGTGA25100.011.4782057
GTCTCTT1865000.011.15519344
GTAGGAC49400.010.8187783
TTCTCGC22650.010.78235342
GTCCTAC53450.010.6576061
TAGACCG4601.8189894E-1210.5185895
TATGGCG47250.010.1470516
TTTTCCG30350.010.14575723
CGTGATT26500.010.13191428
TACACTG90950.010.083845
TTTCCGT30100.09.93833824
GTCCTAA48400.09.9064661
CCGTGAT26850.09.83514527
Produced by FastQC (version 0.11.2)