FastQCFastQC Report
Thu 12 Feb 2015
G111_M12.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG111_M12.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24048050
Sequences flagged as poor quality0
Sequence length50
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCCGAG103000.023.92069644
TCTCCGA138300.017.78349743
TAGGACG55300.015.550344
ATCTCCG168800.014.62450442
AGGACGT61400.013.8262225
GGACGTG60800.013.3474246
GTCCTAC74600.012.234971
GACGTGA42850.012.0096857
GTAGGAC70850.011.5475883
GTCCTAA71950.011.1266451
TTCTCGC40400.011.00096342
TATGGCG83400.010.758916
TTAGGAC79600.010.3334853
TTATACA998150.010.29674744
CGTGATT44950.010.18412228
TTTCTCG45700.010.11073841
TCTCGCC44000.010.09941543
CCGTGAT45700.010.06401427
TACACTG131400.010.0089945
ATGGCGA94650.09.94471717
Produced by FastQC (version 0.11.2)