FastQCFastQC Report
Thu 12 Feb 2015
G111_M13.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG111_M13.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18099680
Sequences flagged as poor quality0
Sequence length50
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCC1704140.9415304579970474No Hit
CCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACC1640920.9066016636758218No Hit
CTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCT981620.542341080063294No Hit
GTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGT967010.5342691141500844No Hit
TAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTA555360.3068341539739929No Hit
AACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAA358100.19784880174677122No Hit
GGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGG332670.18379882959256738No Hit
ACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAAC312620.17272128568018882No Hit
GAAAACTGAAAATCACGGAAAATGAGAAATACACACTTTAGGACGTGAAA275950.15246125898358423No Hit
GTGATTTTCAGTTTTCTCGCCATATTCCAGGTCCTTCAGTGTGCATTTCT245790.13579798095877937No Hit
GGAAAATTTAGAAATGTCCACTGTAGGACGTGGAATATGGCAAGAAAACT241390.1333669987535691No Hit
GGAAAATGAGAAACATCCACTTGACGACTTGAAAAATGACGAAATCACTA238070.13153271218054682No Hit
GGAAAACTGAAAAAGGTGGAAAATTTAGAAATGTCCACTGTAGGACGTGG224750.12417346605022853No Hit
GAAAACTGAAAATCATGGAAAATGAGAAACATCCACTTGACGACTTGAAA223940.12372594432608754No Hit
GTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGCCATATTTC209000.11547165474748726No Hit
GAGAAATACACACTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAG205810.11370919264870982No Hit
GTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCATATTCCAGGT201070.11109036181855148No Hit
GGAAAATGAGAAATACACACTTTAGGACGTGAAATATGGCGAGGAAAACT198690.10977542144391503No Hit
GTCATTTTTCAAGTCGTCAAGTGGATGTTTCTCATTTTCCATGATTTTCA187990.10386371471760827No Hit
GTCCACTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAA184970.10219517693130487No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGGTC8850.033.82798438
AGCCGAT9800.032.9869418
GCCGATG10300.031.3851119
CCGATGA10400.031.29426620
TAAGCCG10800.030.1373816
GCGGTCT9750.030.02525339
CGATGAC10800.029.93201421
AGGCGGT10400.028.5763937
AATTCGG10200.028.24684123
AAGCCGA11650.027.93797717
TCGGTAT10350.027.8473926
CGGTATT10800.027.304227
TCGGGGA10850.026.95758817
TTCGGTA10900.026.43786225
GATGACG12400.026.42662822
CGGTCTC11000.026.4065540
TATTCGT9100.026.1016461
CCTCGGG10200.024.5795915
GATCGGA6050.024.3559441
ATTCGGT11950.024.11180724
Produced by FastQC (version 0.11.2)