FastQCFastQC Report
Thu 12 Feb 2015
G111_M4.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG111_M4.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19973948
Sequences flagged as poor quality0
Sequence length50
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCCGC4250.024.8659433
ACGTCCG5400.019.16240932
GATCGGA7750.013.9048781
CACGACG2502.0918378E-913.1952815
TCTCGTA8400.012.30943743
GTCCGCA8400.012.05677334
CTCGTAT8950.011.552875544
TCCGCAC9200.011.24733235
ATCGGAA10050.011.1602722
ACGACGC3156.4030246E-810.47239316
CGTCTGA13400.08.86245716
CGACGCT3553.6493839E-68.67273717
CCGCACA13350.08.24581736
CGCACAT13050.07.928337
CATCTCG15100.07.8691341
ACACGTC14050.07.66979813
ATCTCGT13550.07.469648442
AGTCACG16550.06.915212628
TCGGAAG16250.06.90218073
ACGTCTG16700.06.8478515
Produced by FastQC (version 0.11.2)