FastQCFastQC Report
Sat 19 Mar 2016
G113-M5_ATCACGAT_AC67REANXX_L002_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG113-M5_ATCACGAT_AC67REANXX_L002_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences250000
Sequences flagged as poor quality0
Sequence length50
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGC6410.25639999999999996TruSeq Adapter, Index 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGATC1050.043.953436
GATCTCG1050.043.953439
GATCGGA1150.042.287651
CACACGT1200.042.1220112
ATCGGAA1200.042.122012
CGTATGC1150.042.0423844
ATCTCGT1100.041.95551740
TCTCGTA1100.041.95551741
TCACGAT1200.040.29867635
ATCACGA1200.040.29867634
ACGATCT1150.040.13136337
ACGTCTG1300.038.91298315
GCACACG1300.038.88185511
TCGTATG1250.038.67899343
CACGTCT1350.037.44927214
ACACGTC1350.037.44178413
CATCACG1300.037.1987833
CTCGTAT1250.036.92085642
TCGGAAG1400.036.104583
CGATCTC1300.035.50082438
Produced by FastQC (version 0.11.2)