FastQCFastQC Report
Sat 19 Mar 2016
G113-M5_ATCACGAT_AC67REANXX_L002_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG113-M5_ATCACGAT_AC67REANXX_L002_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences250000
Sequences flagged as poor quality0
Sequence length50
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG5920.23679999999999998Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG1000.044.010
GTCGCCG1000.044.044
TCGTGTA1050.044.014
GATCTCG1050.044.034
GCGTCGT1000.044.011
TGGTCGC1050.044.042
GATCGGA1200.042.166671
GGTCGCC1100.042.043
CGTCGTG1100.042.012
CGTGTAG1100.042.015
ATCTCGG1150.040.17391635
GTCGTGT1150.040.17391613
CTCGGTG1100.040.037
TCGGAAG1250.038.723
ATCGGAA1250.038.722
AAGAGCG1200.038.5000047
CGGTGGT1200.038.50000439
TCGGTGG1200.038.50000438
AGAGCGT1200.038.5000048
GTGGTCG1250.036.9641
Produced by FastQC (version 0.11.2)