FastQCFastQC Report
Sat 19 Mar 2016
G113-M6_CGATGTAT_AC67REANXX_L002_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG113-M6_CGATGTAT_AC67REANXX_L002_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences250000
Sequences flagged as poor quality0
Sequence length50
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG29241.1696Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTCGG3800.043.4210535
GTCGTGT3750.043.41333413
CGTCGTG3650.043.39726312
GGTCGCC3600.043.3888943
AGCGTCG3550.043.3802810
GCGTCGT3550.043.3802811
GTCGCCG3650.042.7945244
AAGAGCG3550.042.7605637
GATCGGA3950.042.3291131
TCGTGTA3900.042.30769314
CTCGGTG3900.042.30769337
GATCTCG3900.042.30769334
CGTGTAG3800.042.26315715
GAGCGTC3550.042.1408469
TCGGTGG4100.041.85365738
TCTCGGT3950.041.77215236
GTGGTCG3850.041.71428741
TGGTCGC3800.041.6842142
ATCGGAA4050.041.283952
CGGTGGT4200.040.85714339