Basic Statistics
Measure | Value |
---|---|
Filename | G113-M6_CGATGTAT_AC67REANXX_L002_001.R2.fastq |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 250000 |
Sequences flagged as poor quality | 0 |
Sequence length | 50 |
%GC | 43 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG | 2924 | 1.1696 | Illumina Single End PCR Primer 1 (100% over 50bp) |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
ATCTCGG | 380 | 0.0 | 43.42105 | 35 |
GTCGTGT | 375 | 0.0 | 43.413334 | 13 |
CGTCGTG | 365 | 0.0 | 43.397263 | 12 |
GGTCGCC | 360 | 0.0 | 43.38889 | 43 |
AGCGTCG | 355 | 0.0 | 43.38028 | 10 |
GCGTCGT | 355 | 0.0 | 43.38028 | 11 |
GTCGCCG | 365 | 0.0 | 42.79452 | 44 |
AAGAGCG | 355 | 0.0 | 42.760563 | 7 |
GATCGGA | 395 | 0.0 | 42.329113 | 1 |
TCGTGTA | 390 | 0.0 | 42.307693 | 14 |
CTCGGTG | 390 | 0.0 | 42.307693 | 37 |
GATCTCG | 390 | 0.0 | 42.307693 | 34 |
CGTGTAG | 380 | 0.0 | 42.263157 | 15 |
GAGCGTC | 355 | 0.0 | 42.140846 | 9 |
TCGGTGG | 410 | 0.0 | 41.853657 | 38 |
TCTCGGT | 395 | 0.0 | 41.772152 | 36 |
GTGGTCG | 385 | 0.0 | 41.714287 | 41 |
TGGTCGC | 380 | 0.0 | 41.68421 | 42 |
ATCGGAA | 405 | 0.0 | 41.28395 | 2 |
CGGTGGT | 420 | 0.0 | 40.857143 | 39 |