FastQCFastQC Report
Tue 16 Jun 2015
G114-M3_ACAGTG_AC6GPWANXX_L007_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG114-M3_ACAGTG_AC6GPWANXX_L007_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23310599
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGT16150.014.57444711
CGTCGTG17050.013.80512112
CGTGTAG19450.011.87546215
AGCGTCG22350.011.22039610
GATCGGA23550.010.3684271
TCGTGTA22550.010.24291614
TCTCGGT26350.09.18536336
GATCTCG26500.08.96730934
GAGCGTC30000.08.7258269
CGACAAT13550.08.6043381
GTAGATC29600.08.39965931
CGACGAT14850.07.99922941
ACGCGAG13900.07.75466543
ATCTCGG31350.07.72039335
GTCGTGT29000.07.661330713
ATCGGAA32400.07.6042062
GTAGGGA33700.07.376428618
GTGTAGG35700.07.209420716
GTCGCCG35100.07.20728344
CACGCGA13450.07.19645642
Produced by FastQC (version 0.11.2)