FastQCFastQC Report
Tue 16 Jun 2015
G114-M4_GCCAAT_AC6GPWANXX_L007_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG114-M4_GCCAAT_AC6GPWANXX_L007_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24158649
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA15850.013.3461741
TCGTATG17550.011.78399743
GTCGGAT22550.011.3351231
ACGCCAA21850.010.67248932
ACGTTCG15250.010.5303276
TATCTCG19000.010.42140639
CGTATGC19850.010.41868444
CTCGTAT20650.010.01503342
CGTGCGA15250.09.95429138
TCGGATT29200.09.9444312
CGCCAAT22350.09.84332733
CGGTCTA19800.09.66605424
GTCACGC22700.09.40065429
TTACGAC13800.09.2456565
CGTTCGA17350.09.1290097
GTCGCAT12050.08.9603271
GTTACGA14600.08.8897194
CGCTCCG26850.08.84955443
AACGCCG13350.08.73387231
GTCGGAA34900.08.7130161
Produced by FastQC (version 0.11.2)