FastQCFastQC Report
Thu 1 Oct 2015
G114-M5_CAGATC_AC6GPWANXX_L007_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG114-M5_CAGATC_AC6GPWANXX_L007_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38811700
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA36050.019.864621
TCGTATG42850.017.19994443
CGTATGC43650.016.73395544
ACACGTC50400.015.36399913
CGTCTGA52300.014.55335816
ACGTCTG54400.014.3150615
ATCGGAA54150.013.1624082
CTCGTAT55950.013.0156742
CACGTCT65900.012.01728714
GTCGGAT38400.011.7631821
CACACGT70750.011.03810712
TCTCGTA66900.010.62210441
CGGAAGA83450.09.67454
GCCGGAT38600.08.848031
GTCGGTT35650.08.7766941
TCGACCG32550.08.51612327
GTCGAAA17400.08.1046251
TCGGAAG93300.08.063683
CGTGCGA21900.08.03660738
GGCGTAT26000.07.96630051
Produced by FastQC (version 0.11.2)