FastQCFastQC Report
Thu 1 Oct 2015
G114-M5_CAGATC_AC6GPWANXX_L007_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG114-M5_CAGATC_AC6GPWANXX_L007_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38811700
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGTG47600.014.7887112
GTAGATC50350.014.68281631
GATCGGA48750.014.575261
GCGTCGT50750.014.17421511
TCGTGTA49500.013.68777314
GTAGGGA52150.013.66759118
GATCTCG52350.013.491652534
GTCGTGT52350.013.48887113
CGTGTAG51650.013.28841715
TCTCGGT55900.012.91034136
GTGTAGG56150.012.57611416
ATCGGAA61500.011.6966372
AGAGCGT64200.011.6844058
TGTAGGG63950.011.1800317
ATCTCGG64550.011.07806635
AGTGTAG71450.010.65434228
AGCGTCG69100.010.632997510
GAGCGTC70050.010.4573929
GAGTGTA72500.010.04474327
TAATCGA22550.09.9504344
Produced by FastQC (version 0.11.2)