FastQCFastQC Report
Thu 1 Oct 2015
G114-M6_ACTTGA_AC6GPWANXX_L007_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG114-M6_ACTTGA_AC6GPWANXX_L007_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25534682
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA30150.027.4787061
TCGTATG35450.023.52081143
CTCGTAT35850.022.70655642
CGTATGC39200.021.10302444
ACACGTC40850.020.67903113
ACGTCTG43600.019.62693415
CGTCTGA45100.018.87660216
ATCGGAA45700.017.9067212
CACACGT51600.016.88252412
CACGTCT52050.016.69420414
TCTCGTA49700.015.40490341
CGGAAGA60550.014.3506754
GTCGGAT25700.012.6031761
ATCTCGT64750.011.68841640
GCACACG79550.011.22740711
AATCTCG69250.011.0558339
CGGGCTA13000.011.0170611
TTGAATC72250.010.90103436
TCGGAAG79500.010.6809423
GTCGGTT36350.010.2442011
Produced by FastQC (version 0.11.2)