FastQCFastQC Report
Fri 3 Feb 2017
G115-M1_GATCAG_AC6GPWANXX_L007_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG115-M1_GATCAG_AC6GPWANXX_L007_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48165028
Sequences flagged as poor quality0
Sequence length50
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGT19550.010.68964411
CGTCGTG19200.09.967916512
AGCGTCG24250.08.61782710
GTGGTCG26100.07.92361441
TCGTGTA26700.07.662280614
CGTGTAG30500.07.428894515
GTCGTGT29500.07.158723413
TGGTCGC29750.06.655030342
GATCGGA32800.06.57262131
GATCTCG32550.06.218871634
ATCGGAA38050.05.95482352
GAGCGTC39450.05.85501779
GTCGCCG37350.05.7719444
TCGGTGG42300.05.617659638
AGAGCGT42500.05.53835448
CGGTGGT43450.05.21575539
Produced by FastQC (version 0.11.2)