FastQCFastQC Report
Fri 3 Feb 2017
G115-M3_GGCTAC_AC6GPWANXX_L007_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG115-M3_GGCTAC_AC6GPWANXX_L007_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39156978
Sequences flagged as poor quality0
Sequence length50
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC18150.011.27318844
TCGTATG18000.010.87843
CTCGTAT17650.010.84452442
ACGGCTA18950.010.33221632
TCTCGTA17650.09.84718941
CGGCTAC24700.08.10512833
GATCGGA25750.07.8724591
ATCTCGT25250.07.406091740
TCACGGC30650.07.105935630
CGTCTGA30100.07.01610916
ATCGGAA29600.06.8373492
ACACGTC30500.06.707785613
CATCTCG29900.06.40141939
ACGTCTG36500.06.20777515
GTCACGG36200.06.0164929
AGTCACG38450.05.664477328
CACGGCT39450.05.24200531