FastQCFastQC Report
Fri 3 Feb 2017
G115-M3_GGCTAC_AC6GPWANXX_L007_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG115-M3_GGCTAC_AC6GPWANXX_L007_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39156978
Sequences flagged as poor quality0
Sequence length50
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGTG16600.011.26419112
GCGTCGT18350.010.54959511
AGCGTCG20100.09.30276510
GTGGTCG22750.08.99365441
GATCGGA24550.08.4229761
TCGTGTA22800.07.9116714
CGTGTAG26150.07.571136515
GTCGTGT24800.07.539740613
TGGTCGC24300.07.513948442
ATCGGAA30150.07.44221162
GATCTCG27150.07.13161634
GTCGCCG28500.07.101215444
GAGCGTC31200.06.3456649
GGTCGCC31000.06.24469543
TCTCGGT34450.06.19522536
CGGTGGT33100.06.181928639
AGAGCGT33600.05.95787338
TCGGTGG32750.05.912096538
ATCTCGG34250.05.910202535