FastQCFastQC Report
Fri 3 Feb 2017
G115-M4_CTTGTA_AC6GPWANXX_L007_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG115-M4_CTTGTA_AC6GPWANXX_L007_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48110329
Sequences flagged as poor quality0
Sequence length50
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGTG20050.08.8869812
GCGTCGT20950.08.19019311
AGCGTCG24950.06.87712910
GTGGTCG27750.06.818289841
TCGTGTA25450.06.742025414
GATCGGA29650.06.1579621
GATCTCG34600.05.65970934
GTCGTGT31800.05.395740513
CGTGTAG31450.05.385848515
TGGTCGC32500.05.279672642
ATCGGAA36800.05.14084152
Produced by FastQC (version 0.11.2)