FastQCFastQC Report
Fri 3 Feb 2017
G115_M1-M4.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG115_M1-M4.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences194406697
Sequences flagged as poor quality0
Sequence length50
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT78200.09.95969542
TCGTATG82050.09.46537443
CGTATGC92400.08.50057444
TCTCGTA85300.08.46001641
GATCGGA115650.07.2208921
CGTCTGA143700.06.0314916
ATCGGAA140250.06.02300932
ACACGTC150600.05.84287113
ATCTCGT132650.05.80507240
ACGTCTG171100.05.194174315
Produced by FastQC (version 0.11.2)