FastQCFastQC Report
Tue 21 Mar 2017
G115_M3M4.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG115_M3M4.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences87267307
Sequences flagged as poor quality0
Sequence length50
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT36350.09.62353642
TCGTATG37800.09.31240143
CGTATGC40450.08.97449244
TCTCGTA38350.08.37576541
GATCGGA52250.06.9158381
ATCTCGT57850.06.046885540
ACACGTC65850.05.812827613
ACGGCTA39650.05.770354332
CGTCTGA69750.05.550826516
ATCGGAA65900.05.54130272
ACGTCTG79150.05.086152615
Produced by FastQC (version 0.11.2)