Basic Statistics
Measure | Value |
---|---|
Filename | G116-M11_GGCTAC_BC72YLANXX_L008_001.R1.fastq |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 13501322 |
Sequences flagged as poor quality | 0 |
Sequence length | 50 |
%GC | 44 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGC | 1628453 | 12.061433687752947 | TruSeq Adapter, Index 11 (100% over 50bp) |
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACAACTCGTATGC | 31814 | 0.2356361843677234 | TruSeq Adapter, Index 11 (98% over 50bp) |
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACACCTCGTATGC | 31547 | 0.23365860024670176 | TruSeq Adapter, Index 11 (98% over 50bp) |
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATATCGTATGC | 26065 | 0.1930551689678981 | TruSeq Adapter, Index 11 (98% over 50bp) |
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACAGCTCGTATGC | 21578 | 0.15982138637979304 | TruSeq Adapter, Index 11 (98% over 50bp) |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
GATCGGA | 201765 | 0.0 | 43.84012 | 1 |
ATCGGAA | 202205 | 0.0 | 43.679108 | 2 |
TCGGAAG | 203425 | 0.0 | 43.6261 | 3 |
CGGAAGA | 202640 | 0.0 | 43.55306 | 4 |
ACACGTC | 205085 | 0.0 | 43.322315 | 13 |
CGTCTGA | 204930 | 0.0 | 43.31 | 16 |
ACGTCTG | 205410 | 0.0 | 43.279465 | 15 |
GCACACG | 205800 | 0.0 | 43.277603 | 11 |
CACACGT | 205755 | 0.0 | 43.219723 | 12 |
CACGTCT | 206100 | 0.0 | 43.091885 | 14 |
CGTATGC | 200855 | 0.0 | 43.091312 | 44 |
TCTCGTA | 184545 | 0.0 | 42.89594 | 41 |
TCGTATG | 202195 | 0.0 | 42.88641 | 43 |
CTCGTAT | 196275 | 0.0 | 42.877193 | 42 |
ACGGCTA | 201345 | 0.0 | 42.85552 | 32 |
CGGCTAC | 200945 | 0.0 | 42.85436 | 33 |
GTCACGG | 202480 | 0.0 | 42.847748 | 29 |
AGTCACG | 203455 | 0.0 | 42.839157 | 28 |
ATCTCGT | 184710 | 0.0 | 42.81824 | 40 |
TCACGGC | 202360 | 0.0 | 42.80794 | 30 |