FastQCFastQC Report
Wed 10 Jun 2015
G116-M11_GGCTAC_BC72YLANXX_L008_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG116-M11_GGCTAC_BC72YLANXX_L008_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13501322
Sequences flagged as poor quality0
Sequence length50
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGC162845312.061433687752947TruSeq Adapter, Index 11 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACAACTCGTATGC318140.2356361843677234TruSeq Adapter, Index 11 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACACCTCGTATGC315470.23365860024670176TruSeq Adapter, Index 11 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATATCGTATGC260650.1930551689678981TruSeq Adapter, Index 11 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACAGCTCGTATGC215780.15982138637979304TruSeq Adapter, Index 11 (98% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA2017650.043.840121
ATCGGAA2022050.043.6791082
TCGGAAG2034250.043.62613
CGGAAGA2026400.043.553064
ACACGTC2050850.043.32231513
CGTCTGA2049300.043.3116
ACGTCTG2054100.043.27946515
GCACACG2058000.043.27760311
CACACGT2057550.043.21972312
CACGTCT2061000.043.09188514
CGTATGC2008550.043.09131244
TCTCGTA1845450.042.8959441
TCGTATG2021950.042.8864143
CTCGTAT1962750.042.87719342
ACGGCTA2013450.042.8555232
CGGCTAC2009450.042.8543633
GTCACGG2024800.042.84774829
AGTCACG2034550.042.83915728
ATCTCGT1847100.042.8182440
TCACGGC2023600.042.8079430