FastQCFastQC Report
Wed 10 Jun 2015
G116-M11_GGCTAC_BC72YLANXX_L008_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG116-M11_GGCTAC_BC72YLANXX_L008_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13501322
Sequences flagged as poor quality0
Sequence length50
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG159259511.795844880967952Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA1952000.043.7997551
ATCGGAA1945950.043.7644842
GTCGCCG1864850.043.75100744
AGCGTCG1920000.043.72612410
GCGTCGT1921250.043.71958511
CGTCGTG1928350.043.7000512
TCGGAAG1956550.043.6802523
GAGCGTC1919750.043.66889
GTCGTGT1930900.043.63224813
CGGAAGA1934100.043.6185654
TCGTGTA1911950.043.61556214
AAGAGCG1938650.043.5137447
AGAGCGT1936700.043.5010958
TGGTCGC1870950.043.45211842
GGTCGCC1873150.043.42329443
GTGGTCG1891550.043.4217841
GATCTCG1932500.043.40286634
TCGGTGG1938700.043.36962538
TCTCGGT1931550.043.3569936
CGGTGGT1917750.043.35571339
Produced by FastQC (version 0.11.2)