FastQCFastQC Report
Wed 10 Jun 2015
G116-M12_CTTGTA_BC72YLANXX_L008_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG116-M12_CTTGTA_BC72YLANXX_L008_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22957416
Sequences flagged as poor quality0
Sequence length50
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC17700347.7100750363194175TruSeq Adapter, Index 12 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATATCGTATGC442150.19259571721834898TruSeq Adapter, Index 12 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAAACTCGTATGC349850.15239084398697136TruSeq Adapter, Index 12 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAACCTCGTATGC298670.13009739423635483TruSeq Adapter, Index 12 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAAGCTCGTATGC238900.10406223418175634TruSeq Adapter, Index 12 (98% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA2232450.043.8551031
ATCGGAA2234500.043.722232
TCGGAAG2248000.043.628543
CGGAAGA2242600.043.4921464
ACACGTC2266150.043.4154813
CGTCTGA2266850.043.41469216
ACGTCTG2272550.043.40354515
CGTATGC2187700.043.37144
CACGTCT2277000.043.2549714
CACACGT2280750.043.19156312
GCACACG2275350.043.18871311
TCTCGTA1980650.043.17068541
TCGTATG2201350.043.15614743
CTCGTAT2106850.043.13916842
AATCTCG1966650.043.06226739
ATCTCGT1980500.043.04036740
GAACTCC2296700.042.5593221
GTAATCT1983300.042.49892837
AGTCACC2243050.042.43005828
TCTGAAC2320500.042.39672518