Basic Statistics
Measure | Value |
---|---|
Filename | G116-M12_CTTGTA_BC72YLANXX_L008_001.R1.fastq |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 22957416 |
Sequences flagged as poor quality | 0 |
Sequence length | 50 |
%GC | 42 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC | 1770034 | 7.7100750363194175 | TruSeq Adapter, Index 12 (100% over 50bp) |
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATATCGTATGC | 44215 | 0.19259571721834898 | TruSeq Adapter, Index 12 (98% over 50bp) |
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAAACTCGTATGC | 34985 | 0.15239084398697136 | TruSeq Adapter, Index 12 (98% over 50bp) |
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAACCTCGTATGC | 29867 | 0.13009739423635483 | TruSeq Adapter, Index 12 (98% over 50bp) |
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAAGCTCGTATGC | 23890 | 0.10406223418175634 | TruSeq Adapter, Index 12 (98% over 50bp) |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
GATCGGA | 223245 | 0.0 | 43.855103 | 1 |
ATCGGAA | 223450 | 0.0 | 43.72223 | 2 |
TCGGAAG | 224800 | 0.0 | 43.62854 | 3 |
CGGAAGA | 224260 | 0.0 | 43.492146 | 4 |
ACACGTC | 226615 | 0.0 | 43.41548 | 13 |
CGTCTGA | 226685 | 0.0 | 43.414692 | 16 |
ACGTCTG | 227255 | 0.0 | 43.403545 | 15 |
CGTATGC | 218770 | 0.0 | 43.371 | 44 |
CACGTCT | 227700 | 0.0 | 43.25497 | 14 |
CACACGT | 228075 | 0.0 | 43.191563 | 12 |
GCACACG | 227535 | 0.0 | 43.188713 | 11 |
TCTCGTA | 198065 | 0.0 | 43.170685 | 41 |
TCGTATG | 220135 | 0.0 | 43.156147 | 43 |
CTCGTAT | 210685 | 0.0 | 43.139168 | 42 |
AATCTCG | 196665 | 0.0 | 43.062267 | 39 |
ATCTCGT | 198050 | 0.0 | 43.040367 | 40 |
GAACTCC | 229670 | 0.0 | 42.55932 | 21 |
GTAATCT | 198330 | 0.0 | 42.498928 | 37 |
AGTCACC | 224305 | 0.0 | 42.430058 | 28 |
TCTGAAC | 232050 | 0.0 | 42.396725 | 18 |