FastQCFastQC Report
Wed 10 Jun 2015
G116-M13_GAGTGG_BC72YLANXX_L008_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG116-M13_GAGTGG_BC72YLANXX_L008_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27630660
Sequences flagged as poor quality0
Sequence length50
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGATATCTCGTAT7333622.654160269787258TruSeq Adapter, Index 7 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA924200.043.464771
ATCGGAA931100.043.098912
TCGGAAG942000.042.7676933
CGTCTGA948800.042.60698316
CTCGTAT869050.042.59440244
ACACGTC949800.042.58064713
ACGTCTG955900.042.4768815
GTCACGA925450.042.4343529
ACGAGTG915650.042.3817132
CGGAAGA945950.042.3680044
TCTCGTA818400.042.29904643
TATCTCG817500.042.27548241
CACGAGT919700.042.2572531
CGAGTGG916950.042.25446333
TCACGAG927650.042.19383230
AGTCACG942600.042.1639328
CACGTCT961950.042.11141614
GCACACG964800.041.9482711
ATCTCGT824750.041.92524342
CACACGT969000.041.8277312