Basic Statistics
Measure | Value |
---|---|
Filename | G116-M13_GAGTGG_BC72YLANXX_L008_001.R1.fastq |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 27630660 |
Sequences flagged as poor quality | 0 |
Sequence length | 50 |
%GC | 42 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGATATCTCGTAT | 733362 | 2.654160269787258 | TruSeq Adapter, Index 7 (97% over 36bp) |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
GATCGGA | 92420 | 0.0 | 43.46477 | 1 |
ATCGGAA | 93110 | 0.0 | 43.09891 | 2 |
TCGGAAG | 94200 | 0.0 | 42.767693 | 3 |
CGTCTGA | 94880 | 0.0 | 42.606983 | 16 |
CTCGTAT | 86905 | 0.0 | 42.594402 | 44 |
ACACGTC | 94980 | 0.0 | 42.580647 | 13 |
ACGTCTG | 95590 | 0.0 | 42.47688 | 15 |
GTCACGA | 92545 | 0.0 | 42.43435 | 29 |
ACGAGTG | 91565 | 0.0 | 42.38171 | 32 |
CGGAAGA | 94595 | 0.0 | 42.368004 | 4 |
TCTCGTA | 81840 | 0.0 | 42.299046 | 43 |
TATCTCG | 81750 | 0.0 | 42.275482 | 41 |
CACGAGT | 91970 | 0.0 | 42.25725 | 31 |
CGAGTGG | 91695 | 0.0 | 42.254463 | 33 |
TCACGAG | 92765 | 0.0 | 42.193832 | 30 |
AGTCACG | 94260 | 0.0 | 42.16393 | 28 |
CACGTCT | 96195 | 0.0 | 42.111416 | 14 |
GCACACG | 96480 | 0.0 | 41.94827 | 11 |
ATCTCGT | 82475 | 0.0 | 41.925243 | 42 |
CACACGT | 96900 | 0.0 | 41.82773 | 12 |