FastQCFastQC Report
Wed 10 Jun 2015
G116-M13_GAGTGG_BC72YLANXX_L008_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG116-M13_GAGTGG_BC72YLANXX_L008_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27630660
Sequences flagged as poor quality0
Sequence length50
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG7763702.8098134463671878Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGCCG872300.043.60457244
GCGTCGT897450.043.54199211
CGTCGTG899800.043.52849212
AGCGTCG896050.043.52403610
GATCGGA905300.043.5122031
ATCGGAA907950.043.301852
GAGCGTC900450.043.284499
TGGTCGC878450.043.20724542
GGTCGCC878950.043.18884343
TCGTGTA901050.043.12471414
GTCGTGT909400.043.11751613
GTGGTCG885650.043.09552841
AGAGCGT910450.042.9995238
GATCTCG899400.042.9959534
CGTGTAG909150.042.9274815
TCGGAAG917800.042.920383
CGGTGGT899850.042.8717339
TCGGTGG907750.042.8283438
AAGAGCG912500.042.823157
ATCTCGG903300.042.79813435