FastQCFastQC Report
Wed 10 Jun 2015
G116-M14_ACTGAT_BC72YLANXX_L008_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG116-M14_ACTGAT_BC72YLANXX_L008_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22578374
Sequences flagged as poor quality0
Sequence length50
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG4885762.1639113604903524Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGCCG554350.043.6144844
CGTCGTG574600.043.42976812
AGCGTCG571350.043.4069610
GCGTCGT572800.043.39729711
GATCGGA582650.043.258711
TGGTCGC558400.043.18366642
GGTCGCC560250.043.102143
GTGGTCG562350.043.08468241
ATCGGAA584350.043.0676042
GAGCGTC577200.042.9632159
GTCGTGT582700.042.9431713
TCGTGTA578000.042.90888614
GATCTCG571800.042.89071734
CGTGTAG582350.042.78495815
TCGGTGG574550.042.7735338
CGGTGGT570450.042.76460639
TCGGAAG589100.042.762833
TCTCGGT573100.042.69742636
AGAGCGT583950.042.688538
AAGAGCG584350.042.6774647