Basic Statistics
Measure | Value |
---|---|
Filename | G116-M14_ACTGAT_BC72YLANXX_L008_001.R2.fastq |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 22578374 |
Sequences flagged as poor quality | 0 |
Sequence length | 50 |
%GC | 41 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG | 488576 | 2.1639113604903524 | Illumina Single End PCR Primer 1 (100% over 50bp) |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
GTCGCCG | 55435 | 0.0 | 43.61448 | 44 |
CGTCGTG | 57460 | 0.0 | 43.429768 | 12 |
AGCGTCG | 57135 | 0.0 | 43.40696 | 10 |
GCGTCGT | 57280 | 0.0 | 43.397297 | 11 |
GATCGGA | 58265 | 0.0 | 43.25871 | 1 |
TGGTCGC | 55840 | 0.0 | 43.183666 | 42 |
GGTCGCC | 56025 | 0.0 | 43.1021 | 43 |
GTGGTCG | 56235 | 0.0 | 43.084682 | 41 |
ATCGGAA | 58435 | 0.0 | 43.067604 | 2 |
GAGCGTC | 57720 | 0.0 | 42.963215 | 9 |
GTCGTGT | 58270 | 0.0 | 42.94317 | 13 |
TCGTGTA | 57800 | 0.0 | 42.908886 | 14 |
GATCTCG | 57180 | 0.0 | 42.890717 | 34 |
CGTGTAG | 58235 | 0.0 | 42.784958 | 15 |
TCGGTGG | 57455 | 0.0 | 42.77353 | 38 |
CGGTGGT | 57045 | 0.0 | 42.764606 | 39 |
TCGGAAG | 58910 | 0.0 | 42.76283 | 3 |
TCTCGGT | 57310 | 0.0 | 42.697426 | 36 |
AGAGCGT | 58395 | 0.0 | 42.68853 | 8 |
AAGAGCG | 58435 | 0.0 | 42.677464 | 7 |