FastQCFastQC Report
Wed 10 Jun 2015
G116-M15_ATTCCT_BC72YLANXX_L008_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG116-M15_ATTCCT_BC72YLANXX_L008_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25363347
Sequences flagged as poor quality0
Sequence length50
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCCTTTATCTCGTAT1936310.7634284229127962TruSeq Adapter, Index 27 (97% over 44bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA259850.042.70311
ATCGGAA264100.041.889212
CTCGTAT243000.041.1990944
CGTCTGA272050.041.1254316
TCGGAAG271200.040.832783
ACACGTC274500.040.7423513
TCTCGTA228950.040.69407743
ACGTCTG275700.040.6846815
TATCTCG232150.040.30408541
CGGAAGA274500.040.141514
ATCTCGT236250.039.5859642
CACGTCT286900.039.1730914
GCACACG289200.038.70184311
CACACGT292050.038.4145312
GAACTCC326550.034.30216221
GTCACAT322250.033.42899329
CAGTCAC334750.033.16623727
TCTGAAC341450.033.0565118
ACATTCC326450.032.68899532
GTCTGAA344850.032.6540617
Produced by FastQC (version 0.11.2)