FastQCFastQC Report
Wed 10 Jun 2015
G116-M15_ATTCCT_BC72YLANXX_L008_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG116-M15_ATTCCT_BC72YLANXX_L008_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25363347
Sequences flagged as poor quality0
Sequence length50
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG2140170.8438042502828983Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGCCG241950.043.22686844
CGTCGTG250550.042.8989912
AGCGTCG251050.042.8485110
GCGTCGT251000.042.80455411
GATCGGA257050.042.5514681
ATCGGAA257600.042.3426932
TGGTCGC247850.042.21065542
GAGCGTC256400.041.9973349
GTCGTGT257450.041.9113113
GGTCGCC249750.041.8342743
TCGTGTA256450.041.82660714
GATCTCG255900.041.81355334
GTGGTCG252200.041.70996541
CGTGTAG260800.041.2979515
TCGGTGG259700.041.2864738
TCGGAAG265050.041.261853
AGAGCGT262050.041.259458
TCTCGGT259650.041.16728236
ATCTCGG259900.041.08534235
AAGAGCG263750.041.0264637
Produced by FastQC (version 0.11.2)