FastQCFastQC Report
Wed 10 Jun 2015
G116-M2_CGATGT_BC72YLANXX_L008_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG116-M2_CGATGT_BC72YLANXX_L008_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26637024
Sequences flagged as poor quality0
Sequence length50
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGC5380112.0197864446118303TruSeq Adapter, Index 2 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA682750.043.540791
ATCGGAA684300.043.279782
TCGGAAG694000.042.8581853
CGGAAGA693650.042.6768884
CGTCTGA697200.042.65516716
ACACGTC698150.042.62547713
ACGTCTG702500.042.4930215
CGTATGC681650.042.4834244
TCGTATG685450.042.30580543
CTCGTAT666750.042.3050742
CGATGTA664800.042.2826234
ACCGATG673100.042.2774932
TCTCGTA621250.042.2587341
CACCGAT674550.042.24855831
GTCACCG688750.042.18220529
CCGATGT670450.042.1165633
CACGTCT711450.041.92445814
GCACACG711050.041.85215811
TATCTCG622250.041.83387839
TCACCGA687000.041.7870530