FastQCFastQC Report
Wed 10 Jun 2015
G116-M2_CGATGT_BC72YLANXX_L008_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG116-M2_CGATGT_BC72YLANXX_L008_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26637024
Sequences flagged as poor quality0
Sequence length50
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG5651172.1215470617138013Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG657900.043.66066710
GCGTCGT658950.043.64116311
CGTCGTG660650.043.63223612
GTCGCCG644000.043.6212944
GATCGGA667150.043.552931
GAGCGTC661650.043.35679
ATCGGAA669850.043.277392
TGGTCGC648350.043.1898142
GTCGTGT671000.043.17547213
GGTCGCC650350.043.1093543
GTGGTCG654850.043.08115841
TCGTGTA666400.043.02866714
TCGGAAG676000.042.979233
GATCTCG665350.042.968834
AGAGCGT672300.042.947888
CGTGTAG671400.042.9217315
AAGAGCG673350.042.873897
TCGGTGG673250.042.81762338
CGGTGGT668450.042.73660339
CGGAAGA673950.042.7223634
Produced by FastQC (version 0.11.2)