FastQCFastQC Report
Wed 10 Jun 2015
G116-M5_ACAGTG_BC72YLANXX_L008_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG116-M5_ACAGTG_BC72YLANXX_L008_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24509039
Sequences flagged as poor quality0
Sequence length50
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC8618763.516563827737187TruSeq Adapter, Index 5 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATATCGTATGC279210.11392123534504964TruSeq Adapter, Index 5 (98% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA1103250.043.700381
ATCGGAA1104000.043.5271722
TCGGAAG1110950.043.3157163
CGGAAGA1109300.043.122314
CGTCTGA1122100.042.91844616
CGTATGC1100450.042.91422744
ACACGTC1124500.042.87378313
ACGTCTG1127650.042.8710415
TCTCGTA967550.042.7606941
CTCGTAT1047850.042.7327442
TCGTATG1106200.042.72105843
GATCTCG964100.042.6817939
ATCTCGT968550.042.5389740
GCACACG1133850.042.51053611
CACGTCT1136550.042.5043714
CACACGT1137800.042.42094812
AGTGATC990500.041.1156436
GAACTCC1164550.041.09525321
GTCACAC1149050.041.08687229
GTGATCT1001750.040.8075837
Produced by FastQC (version 0.11.2)