FastQCFastQC Report
Wed 10 Jun 2015
G116-M6_GCCAAT_BC72YLANXX_L008_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG116-M6_GCCAAT_BC72YLANXX_L008_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23022259
Sequences flagged as poor quality0
Sequence length50
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC2304181.0008487872541092TruSeq Adapter, Index 6 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA293250.042.6836621
ATCGGAA297250.042.1017652
CGCCAAT287700.041.72506333
CGTCTGA301950.041.68617216
TCGGAAG302950.041.592223
CGTATGC293300.041.5867344
CTCGTAT287900.041.52446742
GTCACGC295200.041.43982729
ACGCCAA291000.041.43328532
TCGTATG295200.041.40120743
TCTCGTA271750.041.36391441
TATCTCG268550.041.30162439
ACACGTC305200.041.24226813
ACGTCTG306400.041.18123615
CGGAAGA306100.040.9413764
AGTCACG304300.040.59810328
ATCTCGT275900.040.58991640
CACGCCA298550.040.50335331
TCACGCC300950.040.48729330
CACGTCT315700.039.8775414
Produced by FastQC (version 0.11.2)