FastQCFastQC Report
Wed 10 Jun 2015
G116-M6_GCCAAT_BC72YLANXX_L008_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG116-M6_GCCAAT_BC72YLANXX_L008_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23022259
Sequences flagged as poor quality0
Sequence length50
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG2422071.052055751783524Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGCCG276750.043.19626644
CGTCGTG285000.043.17931712
AGCGTCG284950.043.0478610
GCGTCGT285400.043.0186111
GATCGGA290200.042.8000451
TGGTCGC280750.042.41144642
GTCGTGT291500.042.36012313
ATCGGAA292350.042.3510782
GTGGTCG283550.042.30370741
GGTCGCC281650.042.26645743
TCGTGTA290750.042.19760514
GAGCGTC291050.042.1154069
GATCTCG289050.042.07927334
TCGGTGG292800.041.7432838
CGTGTAG294500.041.7352415
AGAGCGT294550.041.637378
AAGAGCG295300.041.5316247
TCGGAAG299300.041.487023
TCTCGGT293850.041.48178536
CGGTGGT293000.041.35426339
Produced by FastQC (version 0.11.2)