FastQCFastQC Report
Wed 10 Jun 2015
G116-M7_CAGATC_BC72YLANXX_L008_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG116-M7_CAGATC_BC72YLANXX_L008_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18888553
Sequences flagged as poor quality0
Sequence length50
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC3098921.640633880213058TruSeq Adapter, Index 7 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA386500.043.389431
ATCGGAA389650.042.9827582
CGTATGC383600.042.69077344
TCGGAAG392950.042.672053
TCTCGTA345750.042.67115841
CTCGTAT372150.042.63577742
CGTCTGA396900.042.63526516
TCGTATG385350.042.5941643
ACACGTC399500.042.4128413
ACGTCTG400800.042.29722615
CGGAAGA394600.042.192644
ATCTCGT350250.042.09774840
CATCTCG350450.041.79081339
CACGTCT408450.041.52656614
GCACACG410150.041.3169111
CACACGT410550.041.30879612
GATCATC367050.039.45739736
AGTCACC423300.039.06177528
GAACTCC435850.038.6283421
GTCACCA429750.038.3680329
Produced by FastQC (version 0.11.2)