Basic Statistics
Measure | Value |
---|---|
Filename | G116-M7_CAGATC_BC72YLANXX_L008_001.R2.fastq |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 18888553 |
Sequences flagged as poor quality | 0 |
Sequence length | 50 |
%GC | 42 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG | 324172 | 1.7162352245828465 | Illumina Single End PCR Primer 1 (100% over 50bp) |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
GTCGCCG | 36470 | 0.0 | 43.53187 | 44 |
AGCGTCG | 37490 | 0.0 | 43.436317 | 10 |
CGTCGTG | 37690 | 0.0 | 43.421738 | 12 |
GATCGGA | 38175 | 0.0 | 43.36397 | 1 |
GCGTCGT | 37675 | 0.0 | 43.35146 | 11 |
ATCGGAA | 38245 | 0.0 | 43.13773 | 2 |
TGGTCGC | 36820 | 0.0 | 42.983353 | 42 |
GAGCGTC | 37870 | 0.0 | 42.924965 | 9 |
GTGGTCG | 37220 | 0.0 | 42.87126 | 41 |
GTCGTGT | 38240 | 0.0 | 42.84345 | 13 |
GGTCGCC | 36970 | 0.0 | 42.83129 | 43 |
GATCTCG | 38030 | 0.0 | 42.665806 | 34 |
AGAGCGT | 38200 | 0.0 | 42.663315 | 8 |
TCGTGTA | 37965 | 0.0 | 42.661926 | 14 |
AAGAGCG | 38330 | 0.0 | 42.633038 | 7 |
TCGGTGG | 38290 | 0.0 | 42.600243 | 38 |
TCGGAAG | 38815 | 0.0 | 42.528603 | 3 |
CGGTGGT | 38000 | 0.0 | 42.47362 | 39 |
TCTCGGT | 38130 | 0.0 | 42.46156 | 36 |
CGTGTAG | 38425 | 0.0 | 42.40317 | 15 |