FastQCFastQC Report
Wed 10 Jun 2015
G116-M7_CAGATC_BC72YLANXX_L008_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG116-M7_CAGATC_BC72YLANXX_L008_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18888553
Sequences flagged as poor quality0
Sequence length50
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG3241721.7162352245828465Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGCCG364700.043.5318744
AGCGTCG374900.043.43631710
CGTCGTG376900.043.42173812
GATCGGA381750.043.363971
GCGTCGT376750.043.3514611
ATCGGAA382450.043.137732
TGGTCGC368200.042.98335342
GAGCGTC378700.042.9249659
GTGGTCG372200.042.8712641
GTCGTGT382400.042.8434513
GGTCGCC369700.042.8312943
GATCTCG380300.042.66580634
AGAGCGT382000.042.6633158
TCGTGTA379650.042.66192614
AAGAGCG383300.042.6330387
TCGGTGG382900.042.60024338
TCGGAAG388150.042.5286033
CGGTGGT380000.042.4736239
TCTCGGT381300.042.4615636
CGTGTAG384250.042.4031715