FastQCFastQC Report
Wed 10 Jun 2015
G116-M8_ACTTGA_BC72YLANXX_L008_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG116-M8_ACTTGA_BC72YLANXX_L008_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25539172
Sequences flagged as poor quality0
Sequence length50
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGC3030001.1864127779866944TruSeq Adapter, Index 8 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA396600.043.1438451
ATCGGAA400950.042.594012
CGTATGC395800.042.05368444
TCGGAAG409200.041.901553
TCGTATG398600.041.885543
CGTCTGA409950.041.8729316
CTCGTAT380000.041.77139742
TCTCGTA357150.041.73365441
ACACGTC413750.041.57340613
ACGTCTG415400.041.5353415
CGGAAGA410550.041.511824
AATCTCG353850.041.413539
ATCTCGT363650.040.8785640
CACGTCT424550.040.6142614
GCACACG429200.039.99489211
CACACGT430500.039.94053312
GTCACAC451150.037.04963729
GAACTCC465050.036.8313521
TTGAATC404300.036.098936
TCTGAAC482150.035.80333718
Produced by FastQC (version 0.11.2)