FastQCFastQC Report
Wed 10 Jun 2015
G116-M9_GATCAG_BC72YLANXX_L008_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG116-M9_GATCAG_BC72YLANXX_L008_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23459568
Sequences flagged as poor quality0
Sequence length50
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGC5643952.4058200901227167TruSeq Adapter, Index 9 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA716500.043.38341
CGTATGC704550.043.2676544
ATCGGAA717750.043.1993752
TCGTATG709850.042.9911943
CTCGTAT673400.042.96391342
TCTCGTA622900.042.9496741
CGATCAG700700.042.84076333
CGTCTGA731000.042.82206316
GTCACGA712950.042.7586529
TCGGAAG727500.042.753253
ACGATCA702600.042.71552732
ACACGTC734250.042.67445413
CACGATC705100.042.6607731
ACGTCTG737900.042.6392215
GATCTCG622450.042.6366839
ATCTCGT626900.042.51734540
CGGAAGA727150.042.5104184
TCACGAT712200.042.5103230
AGTCACG724050.042.42512528
CACGTCT745600.042.1162814