FastQCFastQC Report
Wed 10 Jun 2015
G116-M9_GATCAG_BC72YLANXX_L008_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG116-M9_GATCAG_BC72YLANXX_L008_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23459568
Sequences flagged as poor quality0
Sequence length50
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG6039162.574284402850044Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGCCG674200.043.57035444
AGCGTCG697400.043.47513210
GCGTCGT698000.043.45055411
CGTCGTG700650.043.44317212
GATCGGA707750.043.3663021
GAGCGTC700100.043.1849449
ATCGGAA709700.043.1684232
TGGTCGC679150.043.1267842
GTGGTCG683850.043.10757441
GTCGTGT709000.043.0464113
TCGTGTA702350.043.0097514
GATCTCG693950.043.0048734
GGTCGCC681900.042.9637543
TCGGAAG716650.042.8647843
AGAGCGT709550.042.84528
CGTGTAG707400.042.84025615
CGGTGGT695100.042.82608439
TCGGTGG700200.042.78129638
AAGAGCG710700.042.754117
TCTCGGT697100.042.72846636