Basic Statistics
Measure | Value |
---|---|
Filename | G116-M9_GATCAG_BC72YLANXX_L008_001.R2.fastq |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 23459568 |
Sequences flagged as poor quality | 0 |
Sequence length | 50 |
%GC | 43 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG | 603916 | 2.574284402850044 | Illumina Single End PCR Primer 1 (100% over 50bp) |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
GTCGCCG | 67420 | 0.0 | 43.570354 | 44 |
AGCGTCG | 69740 | 0.0 | 43.475132 | 10 |
GCGTCGT | 69800 | 0.0 | 43.450554 | 11 |
CGTCGTG | 70065 | 0.0 | 43.443172 | 12 |
GATCGGA | 70775 | 0.0 | 43.366302 | 1 |
GAGCGTC | 70010 | 0.0 | 43.184944 | 9 |
ATCGGAA | 70970 | 0.0 | 43.168423 | 2 |
TGGTCGC | 67915 | 0.0 | 43.12678 | 42 |
GTGGTCG | 68385 | 0.0 | 43.107574 | 41 |
GTCGTGT | 70900 | 0.0 | 43.04641 | 13 |
TCGTGTA | 70235 | 0.0 | 43.00975 | 14 |
GATCTCG | 69395 | 0.0 | 43.00487 | 34 |
GGTCGCC | 68190 | 0.0 | 42.96375 | 43 |
TCGGAAG | 71665 | 0.0 | 42.864784 | 3 |
AGAGCGT | 70955 | 0.0 | 42.8452 | 8 |
CGTGTAG | 70740 | 0.0 | 42.840256 | 15 |
CGGTGGT | 69510 | 0.0 | 42.826084 | 39 |
TCGGTGG | 70020 | 0.0 | 42.781296 | 38 |
AAGAGCG | 71070 | 0.0 | 42.75411 | 7 |
TCTCGGT | 69710 | 0.0 | 42.728466 | 36 |