FastQCFastQC Report
Mon 3 Jul 2017
G117-M10_GTTTCG_BC72YLANXX_L007_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG117-M10_GTTTCG_BC72YLANXX_L007_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19210986
Sequences flagged as poor quality0
Sequence length50
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTCG18900.033.97980533
CTCGTAT20850.030.55719644
GTTTCGG22950.027.88753534
TCTCGTA20250.027.8699843
CGGAATC19650.027.75804938
TCGGAAT20250.027.69586837
TTTCGGA22750.027.55264535
AATCTCG21700.026.007741
ATCTCGT23650.023.67686842
TTCGGAA27150.022.92537336
GATCGGA32650.021.5705781
GTCACGT31850.021.26870729
ACGTTTC31400.020.87307532
AGTCACG33200.020.66885228
ATCGGAA36900.019.1925532
CGTCTGA39150.018.4264616
ACGTCTG41700.017.29966215
ACACGTC41450.017.13869713
TCGGAAG42300.016.5864523
CGGAAGA45200.015.5708164
Produced by FastQC (version 0.11.2)