FastQCFastQC Report
Mon 3 Jul 2017
G117-M10_GTTTCG_BC72YLANXX_L007_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG117-M10_GTTTCG_BC72YLANXX_L007_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19210986
Sequences flagged as poor quality0
Sequence length50
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGCCG17750.036.81583844
TGGTCGC20550.031.90979242
GTGGTCG21500.031.11569841
GGTCGCC21400.030.53816843
GATCTCG24900.028.1037534
TCGGTGG27200.025.80884238
TCTCGGT28050.025.10520736
CGGTGGT27700.024.70741739
ATCTCGG29300.023.88395135
CTCGGTG30800.022.72077437
GCGTCGT30150.022.25015411
AGCGTCG30400.021.99465210
CGTCGTG31200.021.71295212
GATCGGA34300.020.45211
GAGCGTC35650.018.9405989
ATCGGAA37150.018.7649522
GTCGTGT36600.018.5094513
TCGTGTA36950.018.45327214
CGTGTAG37600.018.42718715
AGAGCGT41750.016.594688
Produced by FastQC (version 0.11.2)